A non−thermal plasma (NTP) system constituted by a radiofrequency generator directly coupled to a treatment probe is described. The NTP generated was analyzed and characterized to determine a plasma safety profile and a spreading technique to be used on heat−sensitive matter. When NTP interacts with surrounding air particles produces reactive oxygen and nitrogen species that exhibit bactericidal and antiseptic effects due to their strong biochemical reactivity. Afterward, the NTP source was validated through bacterial deactivation trials, biological tests and promoting and in vivo wound closure performed in mice. NTP source demonstrated faster wound healing than that performed with conventional treatments... Read more

Electroporation-based therapies (EBT) employ high voltage pulsed electric fields to permeabilize tumor tissue; this results in a detectable impedance change. Impedance characterization with a potentiostat is limited by acquisition time (~10s) as this timeframe is larger than pulse periods used with EBTs (~1s). Here, we develop a rapid electrical impedance spectroscopy (EIS) method for impedance characterization (<1s) of electroporation and thermal effects during irreversible electroporation. A biphasic rectangular chirp signal allows for impedance measurements between 1.8 kHz – 4.93 MHz. For the first time, we show impedance analysis at high frequencies (~1 MHz) can delineate thermal effects from electroporation effects... Read more

Alzheimer’s disease (AD) is a chronic neurodegenerative disease, and its long-term progression prediction is definitely important. The structural Magnetic Resonance Imaging (sMRI) can be used to characterize the cortical atrophy that is closely coupled with clinical symptoms in AD and... Read more