The present work establishes a membraneless, co-laminar flow-based approach to develop a cost-effective microfluidic paper-based analytical device for enzymatic biofuel cell (µPAD-EBFC). The developed µPAD-EBFC supporting the self-capillary fluid transport action, consists of Y-shaped paper microchannel with the fuel (glucose) and oxidant (O2) streaming in parallel over carbon nanotube (CNT) based bucky paper (BP) electrodes modified with biocompatible electrocatalytic enzymes such as Glucose oxidase (GOx) and Laccase without any additional redox cofactor. The electrochemical performance for the modified bioelectrodes i.e. electrocatalytic oxidation and reduction reaction was carried out using Linear Sweep Voltammetry (LSV), Cyclic Voltammetry (CV) and Open Circuit Potential (OCP). The overall performance of µPAD-EBFC was evaluated using polarization studies. Subsequently, the catalytic activity of enzymes on the electrode surface was validated by Scanning Electron Microscope (SEM). This simple and portable µPAD-EBFC can generate the maximum power density to the order of 100 µW/cm2 (600 µA/cm2) at 0.505 V over prolonged durations of around 50 hours. Hence, the presented µPAD-EBFC shows good power density and stability, leading to its strong potential to power miniaturized microelectronics devices and sensors.
