ENGINEERING Sciences for Biology and Medicine (ESBM) is an exciting platform for researchers to discuss a wide range of modern biology and medicine goals. The third edition ESBM 2017 conference was organized by the Tunisian Association of Academic Researchers ATCU, in collaboration with the Technopark of Sfax and the Digital Research Centre of Sfax from May 4–7’ 2017 in Sfax, Tunisia. This conference encourages the scientific interactions among researchers to share their ideas and approaches on all aspects of glitches in biology and medicine.