Differentiation potential

This work aims to investigate the capacity of four gene delivery vehicles to transfect human adult MSCs and evaluate the effects of these delivery vehicles on the cell viability, morphology, and differentiation potential of MSCs. We validate optimal transfection conditions and demonstrate that osteogenic or adipogenic commitment bias depends on different delivery vehicles’ exposure. We further knock out PPARγ gene via CRISPR/Cas9 using each delivery vehicle and indicate various effects of gene carriers on gene function. Our findings suggest that robotic microinjection is a promising method to minimize disruption on MSC function. read more