Lech et al. used a large set of intracranial EEG (iEEG) recordings with direct electrical stimulation (DES) from 45 human epilepsy patients with electrodes implanted both subdurally on the cortical surface and subcortically into the brain parenchyma. Subjects were stimulated in blocks of alternating frequency and amplitude parameters during quiet wakefulness. Stimulating at different frequencies and amplitudes of electric current revealed a persistent pattern of response in the low and the high frequency neural activities. In particular, amplification of the theta (4–7 Hz) and attenuation of the gamma (29–52 Hz) power-in-band was observed with increasing the stimulation parameters. This opposite effect on the low and high frequency bands was found across a network of selected local and distal sites proportionally to the proximity and magnitude of the electric stimulation. Power increase in the theta and decrease in the gamma band was driven by the total electric charge delivered with either increasing the frequency or amplitude of the stimulation current. This inverse effect on the theta and gamma activities was consistently observed in response to different sets of stimulation frequencies and amplitudes, and was the largest for the highest frequency and amplitude parameters. Our results suggest a universal DES effect of amplifying theta and suppressing gamma neural activities in the human brain. These findings reveal the utility of simple power-in-band features for understanding and optimizing the effects of electrical stimulation on brain functions. Determining the basic physiological responses to stimulating the brain electrically at different parameters is fundamental for improving the existing therapies and developing new brain-machine interface approaches to modulate brain functions.